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目的了解安徽省宣城市HIV合并隐孢子虫感染现状及其影响因素。方法采用改良抗酸染色法对宣城市342名HIV感染者的粪便样本进行隐孢子卵囊的检测,同时采用酶联免疫吸附试验(ELISA)法检测血清中特异抗体IgM和IgG,以确定其隐孢子虫感染情况;检测血液标本中CD4+T淋巴细胞及CD8+T淋巴细胞数量;进行问卷调查,探讨合并感染的影响因素。结果隐孢子虫感染率为9.36%,单因素分析结果显示,HIV合并隐孢子虫感染在不同年龄段、不同饮用水源、体内CD4+T淋巴细胞及CD8+T淋巴细胞数量、是否抗病毒治疗及是否服用过驱虫药物的调查对象中其感染率的差异具有统计学意义(P<0.05);多因素Logistic分析表明,合并感染与饮用地表水(OR值0.067,95%CI:0.021,0.215)及CD4+T淋巴细胞≤350个/μl(OR值4.276,95%CI:1.294,14.124)存在相关性。结论安徽省宣城市HIV合并隐孢子虫感染率相对较高,年龄、饮用水源、体内CD4+T淋巴细胞、未进行抗病毒治疗及未服用过驱虫药等因素与感染隐孢子虫有关,应采取重点防治措施。
Objective To understand the status of HIV-associated cryptosporidiosis and its influencing factors in Xuancheng, Anhui Province. Methods The stool samples of 342 HIV-infected persons in Xuancheng were tested for cryptosporidial oocysts by modified acid-fast staining. Serum specific IgM and IgG were detected by enzyme linked immunosorbent assay (ELISA) The infection of sporozoites was detected. The numbers of CD4 + T lymphocytes and CD8 + T lymphocytes in blood samples were detected. Questionnaire investigation was conducted to investigate the influencing factors of coinfections. Results The infection rate of Cryptosporidium was 9.36%. The results of univariate analysis showed that the number of CD4 + T lymphocytes and CD8 + T lymphocytes in HIV-infected patients with Cryptosporidium infection at different ages, different sources of drinking water, and whether antiviral treatment (P <0.05). The multivariate Logistic analysis showed that the combined infection and drinking surface water (OR = 0.067, 95% CI: 0.021, 0.215 ) And CD4 + T lymphocytes ≤350 / μl (OR 4.276, 95% CI: 1.294,14.124). Conclusion The prevalence of HIV infection complicated with Cryptosporidium in Xuancheng, Anhui Province is relatively high. The age, drinking water source, CD4 + T lymphocytes in the body, no antiviral therapy and no antidetroviral drugs are associated with Cryptosporidium infection. Should take the key prevention and treatment measures.