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AIM:To investigate the reversal effect of neferine on multidrugresistance in human gastric carcinoma cell line.METHODS:Cells of a human gastric cancer cells line,SGC7901,and its vincristine(VCR)-resistant variant,SGC7901/VCR,were cultivated with or without neferine and/or VCR.Thecytotoxic effect of VCR was evaluated by the MTT assay.Cellapoptosis induced by VCR was determined by flow cytometry(FCM).The expression of P-glycoprotein(P-gp)and amultidrug-resistance-associated protein(MRP)in cells wasexamined by immunofluorescence and FCM.RESULTS:Neferine at the concentration from 2.5 μmol/L to10 μmol/L had no cytotoxidty to SGC7901 cells,and its variantSGC7901/VCR cells.The IC_(50) of VCR against SGC7901 andSGC7901/VCR cells was 0.059 μg/mL and 2.32 μg/mL,respectively,indicating that SGC7901/VCR cells were 39 timesmore resistant to VCR than its parent SGC7 901 cells.Altertreatment with neferine at concentrations of 2.5,5 and10 μmol/L,the IC_(50) of VCR to SGC7901/VCR cell linedecreased to 0.340,0.128 and 0.053 μg/mL,respectively,thus,increased the chemosensitivity by 6.8-,18.1-and43.8-fold,respectively.SGC7901/VCR cells were apoptosisresistant to VCR.Neferine(2.5,5 and 10 μmol/L)promotedthe VCR-induced apoptosis of SGC7901/VCR cells in a dose-dependent manner.The expressions of P-gp and MRP werestrongly positive in SGC7901/VCR cells,which were significantlydown-regulated alter treatment with neferine(10 μmol/L)for 24 h.CONCLUSION:Neferine reverses multidrug resistance ofhuman gastric carcinoma SGC7901/VCR cells,which maybe associated with the down-regulations of P-gp and MRPexpression in SGC701/VCR cells.
AIM: To investigate the reversal effect of neferine on multidrug resistance in human gastric carcinoma cell line. METHODS: Cells of a human gastric cancer cells line, SGC7901, and its vincristine (VCR) -resistant variant, SGC7901 / VCR, were cultivated with or without neferine and / or VCR.The cytotoxic effect of VCR was determined by the MTT assay. Cellpoptosis induced by VCR was determined by flow cytometry (FCM). The expression of P-glycoprotein (P-gp) and amultidrug-resistance- associated protein ) in cells wasexamined by immunofluorescence and FCM.RESULTS: Neferine at the concentration from 2.5 μmol / L to 10 μmol / L had no cytotoxidty to SGC7901 cells, and its variant SGC7901 / VCR cells.The IC_ (50) of VCR against SGC7901 and SGC7901 / VCR cells were 0.059 μg / mL and 2.32 μg / mL, respectively, indicating that SGC7901 / VCR cells were 39 times more resistant to VCR than its parent SGC7 901 cells. Treatment with neferine at concentrations of 2.5, 5 and 10 μmol / L, the IC_ 50) of VCR to SGC7901 / VCR cell linedecrease , respectively, thus increasing the chemosensitivity by 6.8-, 18.1- and 43.8-fold, respectively. SGC7901 / VCR cells were apoptosis resistant to VCR. Neferine (2.5, 5 and 10 μmol / Promoted the VCR-induced apoptosis of SGC7901 / VCR cells in a dose-dependent manner. These expressions of P-gp and MRP werestrongly positive in SGC7901 / VCR cells, which were significantly reduced-regulated alter treatment with neferine (10 μmol / L) for 24 h. CONCLUSION: Neferine reverses multidrug resistance of human gastric carcinoma SGC7901 / VCR cells, which maybe associated with the down-regulations of P-gp and MRP expression in SGC701 / VCR cells.