论文部分内容阅读
目的观察孕烷X受体(PXR)的基因多态性对肾移植术后患者服用他克莫司浓度/剂量比值(C/D)的影响。方法收集记录57例肾移植受者的血液样本,用Sequenom Mass Array系统基因分型方法对患者进行基因分型,比较不同基因型患者之间他克莫司C/D值的差异。结果在PXR rs3814055各基因型中,CC型、CT型和TT型患者的C/D值分别为(108.87±58.34),(165.23±121.79),(284.79±254.83)ng·mL~(-1)·kg·mg~(-1),差异有统计学意义(P<0.05)。在PXR rs2276707各基因型中,CC型、CT型和TT型患者的C/D值分别为(83.68±28.49),(151.14±114.28),(164.97±129.12)ng·mL~(-1)·kg·mg~(-1),差异有统计学意义(P<0.05)。在PXR rs6785049各基因型中,GG型、AA+AG型患者的C/D值分别为(159.58±131.05),(132.87±100.49)ng·mL~(-1)·kg·mg~(-1),差异无统计学意义(P>0.05)。将PXR rs2276707与PXR rs6785049进行基因型组合分析,在CC-AA基因型组、CT-AG基因型组和TT-GG基因型组患者的C/D值分别为(78.12±20.97),(157.56±117.03),(169.48±136.09)ng·mL~(-1)·kg·mg~(-1),差异无统计学意义(P<0.05)。结论 PXR rs3814055和PXR rs2276707基因多态性对他克莫司的C/D值有影响,PXR rs6785049对C/D值无明显影响。肾移植受者用药前进行PXR rs3814055、PXR rs2276707基因型的检测,有利于指导他克莫司的个体化用药。
Objective To investigate the effect of genetic polymorphisms of pregnane X receptor (PXR) on the concentration / dose ratio (C / D) of tacrolimus in patients after renal transplantation. Methods Blood samples of 57 renal transplant recipients were collected and genotyped by Sequenom Mass Array system genotyping to compare the C / D values of tacrolimus among different genotypes. Results The C / D values of the genotypes CC, CT and TT were (108.87 ± 58.34), (165.23 ± 121.79) and (284.79 ± 254.83) ng · mL -1 in PXR rs3814055 genotypes, · Kg · mg ~ (-1), the difference was statistically significant (P <0.05). Among the genotypes of PXR rs2276707, the C / D values of patients with CC type, CT type and TT type were (83.68 ± 28.49), (151.14 ± 114.28) and (164.97 ± 129.12) ng · mL -1 kg · mg ~ (-1), the difference was statistically significant (P <0.05). Among the genotypes of PXR rs6785049, the C / D values of patients with GG and AA + AG were (159.58 ± 131.05) and (132.87 ± 100.49) ng · mL -1 · kg · mg -1 ), The difference was not statistically significant (P> 0.05). The genotypes of PXR rs2276707 and PXR rs6785049 were analyzed. The C / D values of CC-AA genotype, CT-AG genotype and TT-GG genotype were (78.12 ± 20.97) and (157.56 ± 117.03) and (169.48 ± 136.09) ng · mL -1 · kg · mg -1, respectively, with no significant difference (P <0.05). Conclusion The polymorphisms of PXR rs3814055 and PXR rs2276707 have an impact on the C / D value of tacrolimus, while PXR rs6785049 has no significant effect on the C / D value. Pretreatment of renal transplant recipients PXR rs3814055, PXR rs2276707 genotype detection, is conducive to the individual guidance of tacrolimus medication.