论文部分内容阅读
目的观察葛根素预处理对大鼠肝脏缺血再灌注损伤的防护作用。方法雄性SD大鼠,建立肝脏缺血再灌注模型(HIR)。随机分为假手术组、HIR组和HIR-葛根素预处理组。黄嘌呤氧化酶法、硫代巴比妥酸比色法分别测定肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的变化,同时分析NO含量的改变。结果肝脏缺血再灌注损伤后,与假手术组比较,肝组织中MDA活性及NO含量均显著增加,而SOD活性显著降低;经40mg/kg剂量的葛根素预处理7d后,与模型组相比,肝组织中MDA活性及NO含量显著降低,而SOD活性增高。结论葛根素预处理对大鼠缺血再灌注肝脏损伤有一定的保护作用。
Objective To observe the protective effect of puerarin preconditioning on liver ischemia-reperfusion injury in rats. Methods Male Sprague-Dawley rats were established and hepatic ischemia-reperfusion model (HIR) was established. They were randomly divided into sham operation group, HIR group and HIR-puerarin pretreatment group. Xanthine oxidase method and thiobarbituric acid colorimetry were used to measure the content of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in liver tissue, and the changes of NO content were analyzed. Results Compared with the sham-operated group, the activity of MDA and the content of NO in the hepatic tissue were significantly increased and the activity of SOD was significantly decreased after hepatic ischemia-reperfusion injury. After pretreatment with puerarin at a dose of 40 mg/kg for 7 days, it was compared with the model group. In contrast, MDA activity and NO content in liver tissue were significantly decreased, while SOD activity was increased. Conclusion Puerarin preconditioning has a protective effect on rat liver ischemia-reperfusion injury.