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目的 :研究人胎儿期鼻粘膜的免疫屏障。方法 :应用免疫组化SABC法检测 2 4例 2 0～ 4 0周胎儿鼻粘膜的T淋巴细胞、T4 细胞亚群、B细胞、巨噬细胞、NK细胞及IgA、IgG、补体C3、溶菌酶、HLA -DR抗原等 ,以 8例正常成人鼻粘膜为对照组。结果 :胎儿鼻粘膜中有少量散在的T淋巴细胞、T4 细胞亚群、B细胞 ,数量明显少于对照组 (P <0 .0 1)。但NK细胞、巨噬细胞的数量与对照组差异无显著性 (P >0 0 5) ,胎儿鼻粘膜大部分溶菌酶阳性 ,少部分HLA DR标记阳性 ,IgA、IgG、补体C3 均阴性 ,对照组为阳性。结论 :胎儿期已形成了细胞和体液免疫的基础 ,此时期防御屏障主要是NK细胞、巨噬细胞、溶菌酶。
Objective: To study the immunostimulation of human nasal mucosa during pregnancy. Methods: T lymphocytes, T4 cell subsets, B cells, macrophages, NK cells and IgA, IgG, complement C3, lysozyme of 24 cases of fetal nasal mucosa from 20 to 40 weeks were detected by immunohistochemical SABC method , HLA-DR antigen, 8 cases of normal adult nasal mucosa as control group. Results: Fetal nasal mucosa in a small amount of scattered T lymphocytes, T4 cell subsets, B cells, the number was significantly less than the control group (P <0.01). However, the number of NK cells and macrophages was not significantly different from that of the control group (P> 0.05). Most of the nasal mucosa of the fetus was positive for lysozyme, and a small proportion of HLA DR markers were positive. IgA, IgG and complement C3 were all negative. Group was positive. Conclusion: Fetal immunofluorescence has formed the basis of cellular and humoral immunity during this period mainly NK cells, macrophages, lysozyme.