我们曾报告,狗静注内毒素后,静脉血浆6-酮-PGF_(1α)[前列环素(PGI_2)的水解产物]含量显著增加,注前约为127pg/ml,注后5分～12小时内可高达789～1954pg/ml,这反映休克狗体内PGI_2合成增加。但是,这些PGI_2来源于何种细胞或组织尚不清楚。本文观察了内毒素在体外对巨噬细胞PGI_2代谢的影响。实验用小白鼠腹腔巨噬细胞,加入RPMI1640完全培养基(含15％小牛血清),制成每ml含1×10~6细胞的悬液。将其分放到 We have reported a significant increase in the levels of 6-keto-PGF_ (1α) [PGI_2 hydrolysates] in dogs after intravenous injection of endotoxin into dogs, with an average of 127 pg / ml before injection and 5 to 12 after injection Hours can be as high as 789 ~ 1954pg / ml, which reflects the increased PGI_2 synthesis in shock dogs. However, it is unclear which cell or tissue these PGI_2 originated from. In this paper, the effects of endotoxin on PGI2 metabolism in macrophages were observed in vitro. Experiments with mouse peritoneal macrophages, added RPMI1640 complete medium (containing 15% fetal bovine serum), made per ml containing 1 × 10 ~ 6 cells suspension. Divide it into