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为探索构建禾草内生真菌不感染试验种群的方法,选用醉马草为材料,以150W和300W微波功率分别处理醉马草种子30、40、50和60s,研究不同微波处理强度下内生真菌的致死情况,同时考察微波对醉马草发芽活力的影响。结果表明,150W和300W处理40s以上,醉马草带菌率分别显著降到19.38%、5.22%以下,300W处理60s,醉马草内生真菌可完全致死;150W处理30、40s和300W处理30s后,醉马草的发芽率、发芽势、发芽指数和活力指数与对照(未经微波处理,CK)相比均无显著差异,其他处理下上述指标随处理强度的增加出现不同幅度的下降;150W或300W处理30s和40s的醉马草芽长、根长和生物量与CK无显著差异,其他处理下各指标均受到不同程度的抑制。因此,150W处理40s为剔除醉马草种传内生真菌的最佳方法。本研究为探索无毒醉马草作为饲料及优良牧草的选育提供了技术支持。
In order to explore the method of constructing endophytic fungi that do not infect the experimental population, we studied the effects of microwave irradiation on the endophytic fungi under 30 ℃, 50, 60s with 150W microwave power and 300W microwave power respectively. Fungal death, at the same time to study the microwave on the drunk horse’s germination energy. The results showed that the fungal rates of drunken horsewere significantly reduced to 19.38% and 5.22% respectively after treated with 150W and 300W for 40s, respectively. After treated with 300W for 60s, , The germination rate, germination potential, germination index and vitality index of P. dromedarius had no significant difference compared with the control (without microwave treatment, CK). Under the other treatments, the above indexes decreased with different treatment intensities; Or 300W treatment of 30s and 40s drunken horse’s bud length, root length and biomass and CK no significant difference, the other indicators were under different levels of inhibition. Therefore, the treatment of 40s at 150W is the best method to eliminate Endophytic fungi from Endangered Species. This study provided technical support for exploring non-toxic dromedary as a feed and good forage breeding.