论文部分内容阅读
目的:观察环孢素A(CsA)滴眼剂在角膜组织中的药量及经时过程.方法:1%和0.5%CsA混悬滴眼剂50pl滴人兔眼后在一定时间取角膜和血样,用建立的高效液相色谱法测定含量.结果:1%和0.5%CsA混悬滴眼剂在角膜中的药量和血药浓度基本呈倍数关系.且在滴眼后240min内,角膜中浓度保持在20～52和13～34pg/g,达峰时间均为120min.结论:CsA滴眼后兔角膜中可保持较高浓度,而血液浓度很低,可降低其对肝、肾等的损害.
Objective: To observe the dose and time course of cyclosporin A (CsA) eye drops in corneal tissue.Methods: After 50 μl of 1% and 0.5% CsA suspension eye drops were injected into rabbit eyes, Blood samples were determined by HPLC.The results showed that the dose of 1% and 0.5% CsA suspension in cornea was basically multiples of the plasma concentration, and within 240 minutes after instillation, cornea The concentration was maintained at 20-52 and 13-34 pg / g, and the peak time was 120 min.Conclusion: After CsA eye drops, the rabbit cornea can maintain a high concentration, while the blood concentration is very low, which can reduce its effect on the liver, kidney, etc. The damage.