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目的 研究河南地区汉族人群血清C反应蛋白水平(CRP)及其1059G/C基因多态性与冠心病之间的关系。方法采用全自动生化分析仪测定286例冠状动脉造影患者的血清CRP水平,同时应用聚合酶链反应检测CRP的1059G/C基因多态性,结合冠状动脉造影结果进行分析。结果冠心病组的自然对数转换CRP(InCRP)水平显著高于对照组(P<0.05),以年龄、性别、吸烟、高血压、体重指数和InCRP水平为自变量的Logistic回归分析,InCRP水平(Ro=1.44,P<0.01)和体重指数(OR=1.01,P<0.05)与冠心病独立相关。1059G/C基因多态性等位基因和基因型的分布频率符合Hardy-W einberg平衡(χ2=0.297,P>0.05)。两组的1059G/C基因型和等位基因的分布趋势相同,差异无显著性(P>0.05)。C等位基因携带者的InCRP水平低于GG基因型(0.53±1.01比0.88±1.99 mg/l;P=0.024),而冠状动脉病变程度不受1059G/C基因多态性的影响(χ2=1.374,P>0.05)。结论血清CRP水平升高可能是河南地区汉族人冠心病的独立危险因子;CRP水平可能受其1059G/C基因多态性的影响。
Objective To investigate the relationship between serum C-reactive protein (CRP) and its 1059G / C polymorphism and coronary heart disease in Han nationality in Henan province. Methods The level of serum CRP in 286 patients with coronary angiography was measured by automatic biochemical analyzer. Meanwhile, the 1059G / C polymorphism of CRP was detected by polymerase chain reaction (PCR), and the results of coronary angiography were analyzed. Results The logarithmic conversion of natural logarithm CRP (InCRP) in CHD group was significantly higher than that in control group (P <0.05). Logistic regression analysis of age, sex, smoking, hypertension, body mass index and InCRP level as independent variables, InCRP level (Ro = 1.44, P <0.01) and body mass index (OR = 1.01, P <0.05) were independently associated with coronary heart disease. The distribution frequency of 1059G / C polymorphism alleles and genotypes was in accordance with Hardy-W einberg equilibrium (χ2 = 0.297, P> 0.05). The distribution trend of 1059G / C genotypes and alleles in the two groups showed the same trend with no significant difference (P> 0.05). The level of InCRP in C allele was lower than that of GG genotype (0.53 ± 1.01 vs 0.88 ± 1.99 mg / l; P = 0.024), while the degree of coronary artery disease was not affected by 1059G / C polymorphism (χ2 = 1.374, P> 0.05). Conclusions Elevated serum CRP levels may be an independent risk factor for coronary heart disease in Han nationality in Henan Province. The level of CRP may be affected by the 1059G / C polymorphism.