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目的比较宫颈上皮内瘤变和宫颈癌组织标本中人乳头瘤病毒(HPV)感染的基因型分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对192例宫颈上皮内瘤变和85例宫颈癌组织标本进行23种HPV基因分型检测,并对受检者进行相关资料分析。结果 192例宫颈上皮内瘤变HPV总阳性率为82.29%(158/192),一重感染阳性率为46.88%(90/192),多重感染阳性率为35.42%(68/192);85例宫颈癌HPV总阳性率为88.24%(75/85),一重感染阳性率为65.88%(56/85),多重感染阳性率为22.35%(19/85)。结论基因扩增结合基因芯片检测技术可应用于宫颈病变的组织标本,一次可检测23种HPV基因型别,特异性强,敏感性高,对宫颈癌和癌前病变的防治及其疫苗的研究具有十分重要的意义。
Objective To compare the genotypes of human papillomavirus (HPV) infection in cervical intraepithelial neoplasia and cervical cancer and its clinical significance. Methods Totally 23 HPV genotypes were detected in 192 cases of cervical intraepithelial neoplasia and 85 cases of cervical cancer by gene amplification combined with gene chip technique. The related data were analyzed. Results The positive rates of HPV in 192 cases of cervical intraepithelial neoplasia were 82.29% (158/192), 46.88% (90/192) in one case, and 35.42% (68/192 cases) in multiple case. Cervical The total positive rate of HPV was 88.24% (75/85), the positive rate of primary infection was 65.88% (56/85), and the positive rate of multiple infection was 22.35% (19/85). Conclusion Gene amplification combined with gene chip detection can be applied to tissue samples of cervical lesions, a detection of 23 kinds of HPV genotypes at one time, strong specificity, high sensitivity, prevention and treatment of cervical cancer and precancerous lesions and its vaccine It is of great significance.