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目的：探讨Ｔ细胞亚群变化在乙肝病毒相关肾炎发病中的作用。方法：利用流式细胞仪，检测３０ 例乙肝病毒感染后发生肾损害者周围血ＣＤ４＋ 、ＣＤ８ ＋ 细胞的变化；并对６ 例经肾活检证明为乙肝病毒相关肾炎患者用免疫组化ＡＢＣ法检测肾组织ＣＤ４＋ 、ＣＤ８ ＋ 细胞浸润情况。结果：乙肝病毒感染后发生肾损害组周围血ＣＤ８＋ 细胞明显升高（Ｐ＜ ０．０５）；ＣＤ４＋ ／ＣＤ８ ＋ 明显下降（Ｐ＜ ０．０５），与２４ ｈ 尿蛋白定量呈负相关（ ｒ＝ － ０．５５９６，Ｐ＜ ０．０５）；乙肝病毒相关肾炎患者肾组织ＣＤ４＋ 、ＣＤ８ ＋ 细胞数明显增加（ Ｐ＜ ０．０５）。结论：乙肝病毒感染后发生肾损害者存在周围血Ｔ细胞亚群失衡；不仅特异性体液免疫，而且特异性细胞免疫及非特异性免疫机理也参与乙肝病毒相关肾炎发病
Objective: To investigate the role of T cell subsets in the pathogenesis of hepatitis B virus associated nephritis. Methods: Flow cytometry was used to detect the changes of CD4 +, CD8 + cells in the peripheral blood of 30 patients with hepatitis B virus infection after renal infection. Six patients with hepatitis B virus associated nephritis who were confirmed by renal biopsy were examined by immunohistochemical ABC method Kidney tissue CD4 +, CD8 + cell infiltration. Results: The levels of CD8 + cells in peripheral blood of patients with hepatitis B virus infection were significantly increased (P <0.05), and the levels of CD4 + / CD8 + were significantly decreased (P <0.05) = - 0.5596, P <0.05). The number of CD4 + and CD8 + cells in the kidney of patients with hepatitis B virus associated nephritis increased significantly (P <0.05). CONCLUSIONS: There is an imbalance of T lymphocyte subsets in the peripheral blood of patients with renal impairment after hepatitis B virus infection. Not only specific humoral immunity, but also specific cellular and non-specific immune mechanisms are also involved in the pathogenesis of hepatitis B virus-associated nephritis