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浅表性膀胱肿瘤的患者中,有25%最终病变将侵及肌层。特异性红细胞粘附试验是一种有希望成为预测浅表性膀胱肿瘤发展趋势的方法。但其目前尚存在三大不足之处:一是不能进行定量测定,故实验本身不能标准化,使临床应用大为受限。二是只能用组织切片进行试验。由于细胞间互相附着,使细胞膜不能充分与红细胞发生粘附反应。三是O(H)血型常呈弱反应,易造成假阴性结果。鉴于此种情况,作者介绍了一种ABO(H)同种抗原定量分析的新技术。本方法在特异性红细胞粘附试验的基础上,进行了以下三方面的改进。①制备单个移行上皮细胞或肿
In patients with superficial bladder tumors, 25% of the final lesion will invade the myometrium. Specific erythrocyte adhesion test is a promising method to predict the development of superficial bladder tumors. However, there are still three major shortcomings at present: one is that it can not be quantitatively determined, so the experiment itself can not be standardized and the clinical application is greatly limited. The second is to use tissue sections for testing. As the cells attached to each other, so that the cell membrane can not fully adhere to erythrocyte reaction. Third, O (H) blood type often weak reaction, easy to cause false negative results. Given this situation, the authors introduced a new technique for quantitative analysis of ABO (H) alloantigens. The method based on specific erythrocyte adhesion test, the following three improvements. ① Preparation of a single transitional epithelial cells or swollen