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目的探讨基质金属蛋白酶-26(MMP-26)蛋白与子宫内膜异位症(EM)的相关性。方法将35例EM患者(EM组)和30例对照组按照月经周期时相分为月经早期、月经中期、月经晚期三个时期,用免疫组织化学方法检测EM患者及对照组MMP-26蛋白的表达,同时检测患者血清中MMP-26的浓度,对比分析MMP-26蛋白与EM发病的关系。结果在EM患者正位及异位内膜中均可检测到MMP-26的表达,该蛋白主要定位于子宫内膜腺上皮细胞,小血管壁上也有轻度表达;对照组和EM组两组中在位子宫内膜组织中MMP-26的表达强度具有时空差异,在月经中期表达明显升高,差异具有统计学意义(P<0.05),在EM患者中表达较对照组增强,差异具有统计学意义(P<0.05)。血清中MMP-26蛋白浓度在EM组与对照组中差异无统计学意义(P>0.05),表达水平不具有周期性(P>0.05)。结论 MMP-26蛋白可能与EM的发病相关。
Objective To investigate the relationship between the expression of matrix metalloproteinase-26 (MMP-26) and endometriosis (EM). Methods Thirty-five patients with EM (EM group) and 30 control subjects were divided into three groups: early menstruation, middle menstruation and late menstruation according to the menstrual cycle. Immunohistochemistry was used to detect the expression of MMP-26 in EM patients and control group Meanwhile, the concentration of MMP-26 in serum was detected at the same time, and the relationship between MMP-26 protein and EM was analyzed. Results The expression of MMP-26 was detected in the ectopic and ectopic endometrium of EM patients. The protein mainly localized in the endometrial glandular epithelial cells and was also slightly expressed on the small blood vessel wall. In the control group and the EM group, The expression of MMP-26 in eutopic endometrium had a spatiotemporal difference, which was significantly increased in the middle of menstruation, the difference was statistically significant (P <0.05). The expression of MMP-26 in EM was higher than that in control Significance (P <0.05). Serum MMP-26 protein concentration in the EM group and the control group showed no significant difference (P> 0.05), the expression level does not have periodic (P> 0.05). Conclusion MMP-26 protein may be associated with the pathogenesis of EM.