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目的:利用新疆特有的病例资源,评估维吾尔族妇女宫颈癌患者的免疫水平,探讨宫颈癌患者血浆中白细胞介素(IL-2和IL-10)的表达水平及其与临床病理因素之间的关系。方法:收集宫颈癌50例、癌前病变(CINⅢ)患者20例及对照组(正常或慢性宫颈炎患者)18例的外周血标本,使用酶联免疫吸附实验(ELISA)法检测血浆中细胞因子IL-2和IL-10的表达水平。结果:与对照组相比较,宫颈癌和癌前病变组IL-2的表达水平显著降低(P<0.05),IL-10的表达水平显著升高(P<0.05);在宫颈癌组中,IL-10的表达水平随肿瘤分期逐渐升高(P<0.05);对照组中汉族与维吾尔族人群血浆中IL-2表达水平无显著差异(P>0.05),宫颈癌组中维吾尔族患者血浆IL-2表达水平较汉族患者显著降低(P<0.05)。结论:宫颈癌患者细胞免疫水平低下,体内发生免疫抑制,提示这可能是宫颈癌细胞发生免疫逃逸的机制之一,IL-2可能在维吾尔族宫颈癌发生发展过程中起到重要作用。
OBJECTIVE: To evaluate the immune status of Uighur women with cervical cancer using the special case resources in Xinjiang and to explore the relationship between the expression of interleukin-2 (IL-2) and interleukin-10 (IL-10) and the clinicopathological factors in cervical cancer relationship. Methods: Peripheral blood samples from 50 cervical cancer patients, 20 precancerous lesions (CIN Ⅲ) patients and 18 healthy controls (normal or chronic cervicitis patients) were collected. The levels of cytokines in plasma were detected by enzyme linked immunosorbent assay (ELISA) IL-2 and IL-10 expression levels. Results: Compared with the control group, the expression of IL-2 in cervical cancer and precancerous lesions was significantly decreased (P <0.05), and the expression of IL-10 was significantly increased (P <0.05). In cervical cancer group, The level of IL-10 in the cervical cancer group increased gradually with the tumor stage (P <0.05). There was no significant difference in the plasma levels of IL-2 between the Han and Uygur population in the control group (P> 0.05) IL-2 expression was significantly lower than Han patients (P <0.05). Conclusion: The low level of cellular immunity and immunosuppression in vivo suggest that cervical cancer may play an important role in the immune escape of cervical cancer cells. IL-2 may play an important role in the development and progression of Uighur cervical cancer.