目的观察肝细胞提取物组分Ｓ４对８株肿瘤细胞增殖的影响．方法采用ＭＴＴ比色法研究不同浓度Ｓ４对８株肿瘤细胞不同时相增殖的影响．结果Ｓ４对７４０２，７７２１，７７０３，Ｈｅｐｅ肝癌细胞作用７２ｈ后ＩＣ５０（ｍｇ／Ｌ）分别为３９，４２，９５和６５．对胃腺癌细胞（ＳＧＣ７９０１）、回盲部腺癌细胞（ＨＣＴ８）、肺腺癌细胞（ＧＬＣ８２）作用７２ｈ的ＩＣ５０分别为１４３，９９和５４．对鼻咽癌细胞（ＣＮＥ２）４８ｈ未显示抑制作用．结论Ｓ４抑制７株肿瘤细胞呈现明显的量效与时效关系，在１ｍｇ／Ｌ～１０ｍｇ／Ｌ浓度范围内及２４ｈ～７２ｈ时限内，抑制作用随剂量增加、时间延长而增强 Objective To observe the effect of component S4 of hepatocyte extract on the proliferation of 8 tumor cells. Methods MTT assay was used to study the effects of different concentrations of S4 on the proliferation of 8 tumor cells in different phases. Results The IC50 (mg / L) of S4 on 7402, 7721, 7703 and Hepe hepatocellular carcinoma cells after 3 hours were 39, 42, 95 and 65, respectively. IC50 of gastric adenocarcinoma cells (SGC7901), ileocecal adenocarcinoma cells (HCT8) and lung adenocarcinoma cells (GLC82) for 72h were 143, 99 and 54, respectively. On nasopharyngeal carcinoma cells (CNE 2) 48h showed no inhibitory effect. CONCLUSION: S4 suppresses the proliferation of 7 tumor cells in a dose-dependent and time-dependent manner. The inhibitory effect of S4 is dose-dependent and prolonged in the concentration range of 1 mg / L to 10 mg / L and in the range of 24 h to 72 h