为优化‘砀山酥梨’转基因试管苗的生根条件,解决梨试管苗生根的难题,以‘砀山酥梨’转基因试管苗作为试验材料,研究了基本培养基类型、培养方式、不同的间苯三酚(PG)浓度、不同的IBA浓度和暗培养时间对生根的影响。结果表明:采用两步生根法,试管苗在附加80 mg/L PG和20 mg/L IBA的ASH基本培养基上暗培养7d后转入附加80 mg/L PG的ASH培养基上进行光照培养,生根率达到91.7%。 In order to optimize the rooting conditions of transgenic plantlets of ’Dashan pear’ and solve the problem of rooting in vitro, we used the transgenic plantlets of ’Dashan pear’ as experimental material to study the effects of basic medium types, culture methods, The effects of concentration of phenol (PG), different IBA concentration and dark culture time on rooting were studied. The results showed that on the ASH medium supplemented with 80 mg / L PG and 20 mg / L IBA, the plantlets were darkly cultured for 7 days and then transferred to ASH medium supplemented with 80 mg / L PG for illumination , Rooting rate reached 91.7%.