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人外周血淋巴细胞培养方法的建立,使直接研究人体体细胞突变成为可能,极大地推动了遗传毒理学研究的进展,其中外周血淋巴细胞染色体畸变、微核和姐妹染色单体交换率研究所取得的成就尤为突出。然而,以往的测试方法多停留在定性研究,为此,近年来又建立了外周血抗6-巯基鸟嘌呤(6-TG)突变体测试方法,以期定量测定人体体细胞突变率,本文拟就该领域研究进展做一文献综述。
The establishment of human peripheral blood lymphocyte culture method has made it possible to directly study human somatic cell mutation and greatly promoted the progress of genotoxicity research. Among them, chromosome aberrations of peripheral blood lymphocytes, micronucleus and sister chromatid exchange rate Achievements are particularly prominent. However, in the past, many test methods were reserved for qualitative research. Therefore, in recent years, a test method of peripheral blood 6-mercaptoguanine (6-TG) mutants has been established to quantitatively determine the mutation rate of human somatic cells. The research progress in this field is a literature review.