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目的研究原花青素对氟致雄性小鼠的生殖毒性是否具有交互作用。方法将40只雄性小鼠随机分为4组,即对照组、氟化钠组(NaF,20 mg/kg)、原花青素组(GSP,200 mg/kg)、NaF(20 mg/kg)+GSP(200 mg/kg)组,灌胃5周。颈椎脱臼处死小鼠,分别采集附睾及睾丸样本,检测小鼠睾丸脏器系数、精子计数和精子畸形率。结果各组小鼠体重及睾丸脏器系数比较差异无统计学意义(P>0.05);NaF组精子数量低于对照组(P<0.05),GSP组和NaF+GSP组的精子数量均高于NaF组(P<0.05);NaF组精子畸形率高于对照组(P<0.05),GSP组和NaF+GSP组的精子畸形率均低于NaF组(P<0.05);GSP对NaF染毒雄性小鼠精子数量和精子畸形率存在拮抗作用(P<0.05)。结论原花青素对氟化钠导致的雄性小鼠生殖毒性具有一定的拮抗作用。
Objective To investigate whether procyanidins interact with reproductive toxicity of fluoride-induced male mice. Methods Forty male mice were randomly divided into four groups: control group, sodium fluoride group (20 mg / kg NaF), proanthocyanidins group (GSP 200 mg / kg), NaF (200 mg / kg) group, gavage for 5 weeks. Mice were sacrificed by cervical dislocation, epididymis and testis samples were collected, testis organ coefficient, sperm count and sperm deformity were detected. Results There was no significant difference in body weight and testicular organ coefficient between the two groups (P> 0.05). The number of sperm in NaF group was lower than that in control group (P <0.05), and the number of sperm in GSP group and NaF + GSP group was higher than (P <0.05). The sperm deformity rate in NaF group was higher than that in control group (P <0.05). The sperm deformity rates in GSP group and NaF + GSP group were lower than those in NaF group (P <0.05) Antagonism was observed in sperm count and sperm deformity in male mice (P <0.05). Conclusion Proanthocyanidins antagonize the reproductive toxicity of male mice induced by sodium fluoride.