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我们于1978～1981年从浙江临安和杭州市郊等地聚合草枯萎烂根病株分离的细菌,将其培养性状、形态,革兰氏染色反应、生长适温、生理生化反应等性状,与番茄青枯病原细菌对比,其结果相似。两者并可交互接种。 以聚合草枯萎烂根病原细菌和番茄青枯病原细菌为抗原,按常规注射兔子,分别制得两个抗血清,其效价均在1280～2560之间。为了进一步明确两菌的亲缘关系,将两菌抗血清交互吸附后,再将两菌调换测定,都不能起凝集反应。 试验证明引起聚合草枯萎烂根病的病原细菌与茄科假单胞杆菌[Pseudomonas solanacearum(Smith)Smith]青枯病细菌相同。
From 1978 to 1981, we isolated bacteria from the root blight of herbaceous weeds from Lin’an, Zhejiang and other suburbs of Hangzhou. The characteristics of their traits, morphology, Gram staining, growth temperature and physiological and biochemical reactions were compared with those of tomato Bacterial wilt bacterial contrast, the results are similar. Both can be interactive vaccination. In order to polymerize the sclerophyllous root blight of pathogenic bacteria and the bacterial wilt of tomato, the rabbits were injected according to the conventional method to obtain two antisera respectively, and the titer was between 1280 and 2560. In order to further clarify the genetic relationship between the two bacteria, two bacteria antisera after mutual adsorption, then two bacteria exchange determination, can not agglutination. Tests have shown that the pathogenic bacteria that cause the turfgrass root blight are the same as those of the Pseudomonas solanacearum (Smith) Smith bacterial wilt.