目的　克隆和测定汉滩病毒疫苗生产株 84FLi的全基因组序列 ,了解其分子基础。方法　采用逆转录聚合酶链反应 (RT PCR) ,分节段扩增 84FLi株L、M和S基因片段的cDNA ,直接用PCR产物或克隆入pMD18 T载体后进行测序。结果　 84FLi株L、M、S 3个片段的全基因组序列为6 5 33,36 16和 16 88个核苷酸 ,分别编码 2 15 1,1135 ,4 2 9个氨基酸。A、C、G、T 4种核苷酸分别为 3830 ,2 0 5 0 ,2 5 10和 344 7个 ,GC含量为 38 5 2 % ,AT含量为 6 1 4 8%。序列同源性分析表明 84FLi株与分离自广州的RG9株和分离自安徽的Chen4株高度同源。S片段核苷酸的同源性 99 6 %。与HTNV的国际标准毒株 76 118的 3个片段核苷酸同源性分别为 83 7%、84 0 %和 87 2 % ,氨基酸同源性分别为97 5 %、96 0 %和 97 9%。结论　 84FLi株属于汉滩型病毒 ,并与分离自国内的汉滩病毒同源性较高 ,与中国株Chen4株和RG9株属于同一个亚型 Objective To clone and determine the whole genome sequence of the 84FLi strain of Hantaan virus vaccine and to understand its molecular basis. Methods The cDNAs of L, M and S gene fragments of 84FLi strain were amplified by reverse transcriptase polymerase chain reaction (RT PCR) and sequenced directly using PCR products or cloned into pMD18 T vector. Results The whole genome sequence of L, M and S fragments of 84FLi strain was 6 5 33, 36 16 and 16 88 nucleotides encoding 215, 1135 and 429 amino acids, respectively. The four kinds of nucleotides A, C, G and T were 3830, 20 05, 2510 and 344 7 respectively, with a GC content of 38 5 2% and an AT content of 6 1 48%. Sequence homology analysis showed that the 84FLi strain was highly homologous to the RG9 strain isolated from Guangzhou and the Chen4 strain isolated from Anhui. S fragment Nucleotide homology 99 6%. The nucleotide homologies with the 3 fragments of the international standard strain 76 118 of HTNV were 83.7%, 84.0% and 87.2%, respectively, with amino acid homologies of 97.5%, 96.0% and 97.9% . Conclusion 84FLi strain belongs to Hantaan virus and has high homology with Hantaan virus isolated from China. It belongs to the same subtype with Chinese strains Chen4 and RG9