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目的:研究刺萼龙葵Solanum rostratum Dunal不同萃取物的体外抗氧化活性。方法:采用清除二苯代苦味酰基(DPPH)自由基、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基和铁离子还原/抗氧化能力(FRAP)测定法,对刺萼龙葵不同萃取物的体外抗氧化活性进行评价,并与阳性对照药维生素C(VC)进行比较。结果:3种方法测得的抗氧化活性结果趋势一致,刺萼龙葵总浸膏及各萃取物都有较强的抗氧化活性,其中乙酸乙酯萃取物抗氧化活性最强,对DPPH,ABTS自由基清除率的IC50值分别为38.428,29.175 mg·L-1,对Fe3+还原性相当于3.774 mmol·g-1的FeSO4当量,弱于VC(IC50值分别为10.551,8.570 mg·L-1,FeSO4当量为22.795 mmol·g-1);其次是正丁醇萃取物,对DPPH,ABTS自由基清除率的IC50值分别为53.142,57.895 mg·L-1,对Fe3+还原性相当于1.936 mmol·g-1的FeSO4当量。刺萼龙葵不同萃取物和VC体外抗氧化活性的强弱顺序为:VC>乙酸乙酯萃取物>正丁醇萃取物>总浸膏>石油醚萃取物>剩余水部位,随着各萃取物浓度的增加,抗氧化能力增强。结论:刺萼龙葵乙酸乙酯萃取物、正丁醇萃取物及总浸膏均有较强的抗氧化活性,本研究为恶性杂草刺萼龙葵的开发利用提供理论依据。
Objective: To study the antioxidant activity of different extracts of Solanum rostratum Dunal in vitro. Methods: The radical scavenging activity of DPPH and 2,2 - azonia - di (3 - ethyl - benzothiazole - 6 - sulfonic acid) / Antioxidant capacity (FRAP) assay, the antioxidant activity of different extracts of Solanum nigrum were evaluated in vitro and compared with the positive control vitamin C (VC). Results: The results of the three methods showed the same trend of antioxidant activity. The total extract of Solanum lycopersicum and its extracts had strong antioxidative activity. Among them, the extract of ethyl acetate had the strongest antioxidant activity, The IC50 values of ABTS free radical scavenging rate were 38.428 and 29.175 mg · L-1, respectively, which were equivalent to Fe3 + reduction of 3.774 mmol · g-1 FeSO4 equivalent, but lower than that of VC (IC50 values were 10.551 and 8.570 mg · L- 1, FeSO4 equivalent of 22.795 mmol · g-1), followed by n-butanol extract, the IC50 values of radical scavenging for DPPH and ABTS were 53.142 and 57.895 mg · L-1, respectively, and the reducibility for Fe3 + was 1.936 mmol FeSO4 equivalent of g-1. The order of the antioxidant activity of different extract and VC of Calyx Carya in vitro is as follows: VC> ethyl acetate extract> n-butanol extract> total extract> petroleum ether extract> residual water fraction, Increased concentration of substances, antioxidant capacity increased. CONCLUSION: Ethyl acetate extract, n-butanol extract and total extract of Calyx japonica have strong antioxidative activity. This study provides a theoretical basis for the development and utilization of Malvaceae Calyx.