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目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中MMP-12的表达及临床意义。方法应用逆转录-聚合酶链反应(RT-PCR)检测12例Ⅰ期和26例Ⅱ、Ⅲ期非小细胞肺癌及癌旁组织中MMP-12 mRNA的表达;Western blot检测部分标本中MMP-12蛋白表达。结合临床病理资料,比较MMP-12 mRNA表达与非小细胞肺癌临床病理特征的关系。结果MMP-12 mRNA在38例癌旁组织中无表达,在12例Ⅰ期和26例Ⅱ、Ⅲ期癌组织中呈不同程度的表达,半定量均值分别为(0.551±0.140)和(1.082±0.424),其差异有统计学意义(P=0.001);MMP-12蛋白在癌旁组织中无表达,而在Ⅰ期癌组织中低表达,在Ⅱ、Ⅲ期癌组织中高表达。结论MMP-12在非小细胞肺癌组织中高表达,并与淋巴结的转移存在显著相关性,表明MMP-12基因转录水平异常在非小细胞肺癌的发生、发展中起了重要作用。
Objective To investigate the expression of MMP-12 in non-small cell lung cancer (NSCLC) and its clinical significance. Methods RT-PCR was used to detect the expression of MMP-12 mRNA in 12 cases of stage I and 26 cases of stage II and stage III non-small cell lung cancer and its adjacent tissues. Western blot was used to detect the expression of MMP- 12 protein expression. Combined with clinicopathological data, MMP-12 mRNA expression was compared with clinicopathological features of non-small cell lung cancer. Results There was no expression of MMP-12 mRNA in 38 paracancerous tissues. The expression of MMP-12 mRNA was found in 12 cases of stage Ⅰ and in 26 cases of stage Ⅱ and stage Ⅲ tumors, with semi-quantitative mean values of (0.551 ± 0.140) and (1.082 ± 0.424), the difference was statistically significant (P = 0.001); MMP-12 protein was not expressed in adjacent tissues, but low expression in stage Ⅰ cancer tissues, and high expression in stage Ⅱ and Ⅲ cancer tissues. Conclusions MMP-12 is highly expressed in non-small cell lung cancer tissues and has a significant correlation with lymph node metastasis, indicating that the abnormal transcription of MMP-12 gene plays an important role in the development of non-small cell lung cancer.