论文部分内容阅读
目的 :研究碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor ,bFGF)拮抗大鼠心肌细胞缺氧 /复氧(hypoxia/reoxygenation ,H/R)损伤的机制。 方法 :H/R处理的大鼠心肌细胞的孵育液中 ,对照组不加任何处理因素 ,其它各组分别加bFGF(10 μg·L-1)、bFGF与丝裂素活化蛋白激酶 (mitogen activatedproteinkinase,MAPK)抑制剂PD980 5 9(5 0 μmol·L-1)、蛋白激酶C(proteinkinaseC ,PKC)抑制剂H7(40 μmol·L-1)、一氧化氮合酶 (nitricoxidesynthase ,NOS)抑制剂左旋硝基精氨酸甲酯 (NG nitro L argininemethylester,L NAME ,5 0 μmol·L-1)。孵育完毕 ,测定细胞活力、ATP含量及孵育液中乳酸脱氢酶 (lactatedehydrogenase,LDH)含量。 结果 :H/R组心肌细胞活力较对照组降低 32 % (P <0 .0 1) ,细胞ATP含量减少 5 8% (P <0 .0 1) ,孵育液中LDH活性增加 5 .8倍 (P <0 .0 1) ;bFGF组心肌细胞活力较H/R组增高 17% (P <0 .0 1) ,细胞ATP含量增加 45 % (P <0 .0 1) ,孵育液中LDH活性降低 31% (P <0 .0 1) ,细胞PKC、MAPK活性分别增加 32 % (P <0 .0 1)和 10 % (P <0 .0 5 )。PD980 5 9、H7、PD980 5 9+H7及L NAME各组心肌细胞活力较bFGF组分别降低 13%、17%、2 4%和 2 7% (均P <0 .0 1) ,细胞ATP含量分别减少 2
Objective: To investigate the mechanism of basic fibroblast growth factor (bFGF) antagonizing hypoxia / reoxygenation (H / R) injury in rat cardiomyocytes. Methods: H / R-treated rat cardiomyocytes were incubated in the control group without bFGF (10 μg · L -1), bFGF and mitogen activated protein kinase , MAPK inhibitor PD98059 (50μmol·L-1), protein kinase C (PKC) inhibitor H7 (40μmol·L-1) and nitric oxide synthase (NOS) inhibitor NG nitro L arginine methylester (L NAME, 50 μmol·L -1). After incubation, cell viability, ATP content and lactate dehydrogenase (LDH) content in the incubation solution were determined. Results: Compared with the control group, the viability of cardiomyocytes in H / R group decreased by 32% (P <0.01), the ATP content in cells decreased by 58% (P <0.01), and the LDH activity increased by 5.8 times (P <0.01). Compared with H / R group, the viability of cardiomyocytes in bFGF group increased by 17% (P <0.01) and the ATP content in cells increased by 45% (P <0.01) The activity of PKC and MAPK increased by 32% (P <0. 01) and 10% (P <0. 05) respectively when the activity was decreased by 31% (P <0.01). The viability of cardiomyocytes in PD9805 9, H7, PD9805 9 + H7 and L NAME groups were decreased by 13%, 17%, 24% and 27% (all P <0.01) Decrease by 2 respectively