通过斑点杂交和Ｓｏｕｔｈｅｒｎ印迹技术，首次从已构建的裴氏着色真菌基因组ＤＮＡ库中筛选出种特异性ＤＮＡ探针。首先借助斑点杂交技术，筛选出与紧密着色真菌、皮炎万氏霉、疣状瓶霉、卡氏枝孢霉和甄氏外瓶霉及细菌、人白细胞等无同源性的二个ＤＮＡ片段；再经Ｓｏｕｔｈｅｒｎ印迹分析，确认其中的Ｂｆ片段（约３．１ｋｂ）具有良好的裴氏着色真菌种特异性，并初步显示具有多态性特征。 Through spot blotting and Southern blotting techniques, species-specific DNA probes were first screened from the constructed genomic DNA library of the Francoisella coloris. Firstly, two DNA fragments with no homology to the closely-colored fungus, V. dermatitis, P. vulgaris, C. claibneri, and E. exigua, bacteria, and human leukocyte were screened by the dot blotting technique. After Southern blotting analysis, it was confirmed that the Bf fragment (about 3.1 kb) had good species specificity for the Gibsonella species, and it initially showed polymorphism.