本文叙述了激光扫描共聚焦显微镜的光学原理，以花粉颗粒为例，利用激光扫描共聚焦显微镜对生物组织进行连续“光学切片”断层扫描，并重建其三维结构。结果显示重建后的图像为高分辨率的三维原色图像，可沿着Ｘ、Ｙ、Ｚ轴旋转或其它任意角度显示。 This article describes the optical principle of laser scanning confocal microscopy. Taking pollen grains as an example, a continuous “optical slice” tomography scan of biological tissue is performed using a laser scanning confocal microscope and its three-dimensional structure is reconstructed. The results show that the reconstructed image is a high-resolution, three-dimensional primary image that can be rotated along the X, Y, Z axes or at any other angle.