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为探讨坛紫菜(Pyropia haitanensis)种间杂交的可行性,进一步选育坛紫菜新品系,以野生型坛紫菜作为母本,以采自印度的Pyropia radi作为父本进行种间杂交,并以亲本叶状体细胞的液泡大小与色素体的大小和颜色作为遗传标记:母本的细胞内液泡较小,具一个较大的色素体,颜色偏红;父本的细胞内具有一个很大的液泡,但色素体较小,颜色偏褐带黄。杂交实验结果如下:依据液泡体积、色素体的大小和颜色区分,在F1叶状体中出现了6种类型的细胞:2种亲本细胞类型,4种杂交重组细胞类型,它们分别为母本型(Ph),父本型(Pr),似母本型(Ph’,液泡大小与Ph相同,但色素体偏黄),似父本Ⅰ型(Pr’,液泡比Pr小,且色素体偏红),似父本Ⅱ型(Pr″,液泡大小介于Pr和Pr’之间,且色素体颜色比Pr’更浅),似父本Ⅲ型(Pr,液泡大小与Pr相同,但色素体稍黄)。这6种细胞在F1叶状体上出现分离,并呈块状直线排列。在F1叶状体中出现大量由2~4个色块构成的颜色嵌合体和少数单色的叶状体。上述结果证实这两种紫菜的种间杂交是成功的,并且F1叶状体是成活和可育的,为今后选育种间杂交新品种奠定了基础。
In order to explore the feasibility of interspecific cross between seaweed (Pyropia haitanensis) and new breeding seaweed new seaweed cultivars, wild-type seaweed (Porphyra yezoensis) was used as the female parent and Pyropia radii collected from India as the male parent for interspecific hybridization. The vacuolar size of frond cells and the size and color of the pigment body serve as genetic markers: the female cytoplasm has smaller vacuoles, a larger pigment body, and a reddish color; the paternal cell has a large vacuole , But the pigment body is smaller, the color is brownish yellowish. The results of the hybridization experiment are as follows: According to the vacuole volume, the size and color of the pigment body, six types of cells appeared in the F1 frond: two parental cell types and four hybrid recombinant cell types, which were female type (Ph), paternal (Pr), paramyxoid (Ph ’, vacuolar size same as Ph but yellow pigment) Red), resembles a paternal type II (Pr ", vacuolar size between Pr and Pr ’, and a colorant lighter in color than Pr’ Pigment body slightly yellow.) The six kinds of cells appear in the F1 frond separation, and the block linear arrangement in the F1 fronds appear a large number of 2-4 color blocks formed by a color mosaic and a small number of monochrome Of the fronds.The above results confirm that the cross between the two species of laver is successful, and the F1 fronds are viable and fertile, which will lay the foundation for the future selection of new hybrid varieties.