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从绿脓杆菌(Pseudomonas aeruginosa)PAK菌株的染色体DNA构建的基因文库中筛选到一 基因片段与绿脓杆菌的抗性相关。经测序证明该片段包含了绿脓杆菌基因组中PA4293所编码 序列,进一步实验证明与它相邻的另一段827bp的基因也与绿脓杆菌的抗药性有关。上述2基 因分别命名为pprA和pprB。以绿脓杆菌PAK菌株为出发菌株,经过新霉素耐受培养得到抗生素 抗性菌株PAK1-3,该菌株对氨基糖苷类抗生素的抗性明显增强。经PCR方法扩增上述基因并克 隆至PAK1-3菌株中,可引起该菌株对氨基糖苷类抗生素的敏感。又经pprB基因转入临床分离 得到的致病性绿脓杆菌中,导致部分绿脓杆菌对氨基糖苷类抗生素的敏感性增强。pprA和pprB 很可能是一组与绿脓杆菌抗药性相关的双分子调节系统,并与细胞膜的通透性有关。
A gene fragment was screened from a gene library constructed from the chromosomal DNA of Pseudomonas aeruginosa PAK strains and is associated with the resistance of Pseudomonas aeruginosa. Sequencing proved that the fragment contained the sequence of PA4293 in the genome of Pseudomonas aeruginosa. Further experiments showed that another 827 bp gene adjacent to it was also related to the resistance of Pseudomonas aeruginosa. The above two genes are named pprA and pprB, respectively. The strain Pseudomonas aeruginosa PAK was taken as a starting strain, and the antibiotic resistant strain PAK1-3 was obtained after neomycin resistance cultivation. The resistance of the strain to aminoglycoside antibiotics was significantly enhanced. Amplification of the above genes by PCR and cloning into PAK1-3 strain can cause the strain to be sensitive to aminoglycoside antibiotics. After the pprB gene was transferred into the pathogenic Pseudomonas aeruginosa isolated clinically, the sensitivity of some Pseudomonas aeruginosa to aminoglycoside antibiotics was enhanced. It is likely that pprA and pprB are a group of bimolecular regulatory systems associated with Pseudomonas aeruginosa resistance and are associated with cell membrane permeability.