免疫 Ｐ Ｃ Ｒ 为一种高敏感度检测抗原的新技术， 操作程序大多沿袭 Ｅ Ｌ Ｉ Ｓ Ａ 方法． 用戊二醛作连接剂， 将蛋白质高效率包被在普通 Ｐ Ｃ Ｒ 管内壁， 使免疫及 Ｐ Ｃ Ｒ 反应用普通 Ｐ Ｃ Ｒ 仪得以在管中连贯地进行． 实验结果表明，标准曲线的线性关系好， 与 Ｅ Ｌ Ｉ Ｓ Ａ 方法比较敏感度高出约１０ ５ ． 这一改良法的建立， 可望促进免疫 Ｐ Ｃ Ｒ 的普及应用 Immune P C R is a new technique for high-sensitivity detection of antigens. Most of the procedures follow the E L I S A method. Using glutaraldehyde as the linker, the protein was efficiently coated on the inner wall of an ordinary P C R tube to allow immunization and P C R reactions to be performed consistently in the tube using a conventional P C R instrument. The experimental results show that the standard curve has a good linear relationship, which is about 105 higher than the E L I S A method. The establishment of this improved law is expected to promote the popularization and application of immune P C R