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目的 客观评价PCR技术在人类免疫缺陷病毒(human immune-deficiency virus,HIV)检测中的性能,了解聚合酶链技术(polymerase chain reaction,PCR)PCR技术作为艾滋病筛查方法的临床应用价值。方法 以diagnosis Tests(诊断性试验),AIDS(艾滋病),PCR(聚合酶链反应),HIV(人类免疫缺陷病毒)为检索词,对1991年～2001年的MEDLINE和CBM进行了回顾性机检,并根据国际公认标准对纳入文献进行分析。结果 共检索到567篇论著性文章,纳入53篇。其中与金标准进行对比的有47％,同时计算了和能计算出敏感度、特异度、预测值等统计学指标的文章数量分别为25％、23％和23％。没有文章计算似然比。结论 PCR技术在HIV检测特别是筛查中的应用还处在探索阶段,有待于提高研究方案的总体设计水平。
Objective To objectively evaluate the performance of PCR in the detection of human immunodeficiency virus (HIV) and to understand the clinical value of polymerase chain reaction (PCR) as a screening method for AIDS. Methods MEDLINE and CBM from 1991 to 2001 were retrospectively reviewed with the diagnostic tests, AIDS, PCR and HIV (human immunodeficiency virus) And analyze the included documents based on internationally accepted standards. Results A total of 567 dissertations were collected and included 53 articles. Among them, 47% were compared with the gold standard, and the number of articles that calculated and calculated the statistical indexes such as sensitivity, specificity and forecast value were 25%, 23% and 23% respectively. No article to calculate the likelihood ratio. Conclusion The application of PCR in HIV testing, especially in screening is still in the exploratory stage, and the overall design level of research programs needs to be improved.