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目的研究胃癌组织中细胞周期素D1(cyclin D1)的表达,并分析其与细胞周期的关系。方法采用Western blot和流式细胞术检测33例胃癌组织及其相应正常胃黏膜周期素D1表达和细胞周期分布。结果胃癌组织周期素D1阳性率63.6%,高于正常胃黏膜的21.1%(P<0.01)。周期素D1阳性组胃癌G1/G0期细胞比例为(42.2±1.5)%,显著低于周期素D1阴性组的(64.3±4.8)%(P<0.01);周期素D1阳性组胃癌S期细胞比例为(55.1±1.8)%,显著高于周期素D1阴性组的(34.5±4.9)%(P<0.01)。结论胃癌细胞高表达细胞周期素D1预示癌细胞具有高增殖活性。周期素D1在胃癌发生、发展过程中可能起着重要的作用。
Objective To study the expression of cyclin D1 in gastric carcinoma and its relationship with cell cycle. Methods Western blot and flow cytometry were used to detect the expression of cyclin D1 and the cell cycle distribution in 33 cases of gastric carcinoma and their corresponding normal gastric mucosa. Results The positive rate of cyclin D1 in gastric cancer was 63.6%, which was higher than that of normal gastric mucosa (21.1%, P <0.01). The proportion of cells in G1 / G0 phase in the cyclin D1 positive group was (42.2 ± 1.5)%, which was significantly lower than that in the cyclin D1 negative group (64.3 ± 4.8)% (P <0.01) (55.1 ± 1.8)%, which was significantly higher than that of the cyclin D1 negative group (34.5 ± 4.9%) (P <0.01). Conclusion High expression of cyclin D1 in gastric cancer cells indicates that cancer cells have high proliferative activity. Cyclin D1 may play an important role in the occurrence and development of gastric cancer.