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目的探讨定量研究视网膜色素上皮（ＲＰＥ）细胞吞噬视杆细胞外节（ＲＯＳ）膜盘和纤维连接蛋白（ＦＮ）的方法。方法体外培养人胎儿ＲＰＥ细胞，以荧光标记的聚苯乙烯微球、ＲＯＳ、ＦＮ或ＦＮ包被的荧光微球作为吞噬标记物，建立ＲＰＥ细胞吞噬模型，使用流式细胞仪定量检测ＲＰＥ细胞的吞噬指数。结果经３ｈ孵育，ＲＰＥ细胞对荧光标记的ＲＯＳ（５×１０６／孔）和ＦＮ（４ｍｇ／Ｌ）的吞噬指数分别为５９．９５％±２．０５％和３９．６％±１．１５％；ＲＰＥ细胞对ＦＮ包被微球的吞噬指数明显高于未包被微球的吞噬指数（Ｐ＜０．０５）。结论该研究建立的吞噬模型及定量检测方法简单、易行、可靠，可进一步用于ＲＰＥ细胞吞噬ＲＯＳ和ＦＮ的机理的研究。
Objective To investigate the method of quantitatively studying the phagocytosis of rod membrane and fibronectin (FN) in retinal pigment epithelium (RPE) cells. Methods Human fetal RPE cells were cultured in vitro. Fluorescently labeled polystyrene microspheres, fluorescent microspheres coated with ROS, FN or FN were used as phagocytosis markers to establish phagocytic model of RPE cells. Flow cytometry was used to detect RPE cells Swallow the index. Results Phagocytic index of fluorescent labeled ROS (5 × 106 / well) and FN (4mg / L) in RPE cells were 59.95% ± 2.05% and 39.6% ± 1.15% ; Phagocytic index of RPE cells to FN coated microspheres was significantly higher than that of uncoated microspheres (P <0.05). Conclusion The phagocytosis model and quantitative detection method established in this study is simple, easy and reliable and can be used to study the mechanism of phagocytosis of ROS and FN in RPE cells.