Objective: The purpose of the study was to investigate the relationship between the expression of estrogen re-ceptor (ER), progestogen receptor (PR), human epidermal growth factor receptor (Her-2), Ki-67 and the ef ect of neoadjuvant chemotherapy in breast cancer. Methods:The expression of ER, PR, Her-2 and Ki-67 in 45 breast cancers which received neoadjuvant chemotherapy was detected by immunohistochemistry. Results:The ef ective rates in ER negative and PR negative groups were higher than those in ER positive and PR positive groups (83.3%vs 59. 4%, 82.4%vs 60.6%). There was no significant dif erence of the ef ective rate between Her-2 overexpressed group and Her-2 non-overexpressed group (81.8%vs 64.1%), and the same thing happened between Ki-67 negative group and Ki-67 positive group (67.7%vs 63.2%). Conclusion:In the patients with breast cancer, ER, PR negative ones were more sensitive to neoadjuvant chemotherapy. These patients may get more benefits from chemotherapy. ER, PR could be feasible markers for predicting the ef ective rate of neoadjuvant chemotherapy.