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目的:探讨姜黄素(Cur)与格列本脲(Gli)联用对2型糖尿病大鼠糖代谢的影响及其机制。方法:复制大鼠2型糖尿病模型,观察Cur(100 mg·kg-1·d-1ig)×5 d单用及与Gli(1 mg·kg-1·d-1ig)×5 d联合应用对2型糖尿病大鼠空腹血糖、血浆胰岛素、胰岛素敏感性指数、肝糖原、肝毒性相关指标以及CYP3A活性的影响。结果:Cur能进一步增强Gli的降糖作用,应用Cur后比单用Gli进一步降低空腹血糖(22%至69%),上调胰岛素及肝糖原水平(49%、19%至126%、81%)。Cur可显著下调肝损伤指标,对肝脏具有保护作用。Cur单用或与Gli联用均显著抑制CYP3A活性。结论:Cur可使Gli降糖效应增强,其机制可能与其抑制Gli代谢酶-CYP3A活性,使Gli代谢延缓,致使其降糖效应增强有关。
Objective: To investigate the effect of curcumin and glyburide (Gli) on glucose metabolism in type 2 diabetic rats and its mechanism. Methods: The model of type 2 diabetes mellitus was reproduced in rats. The effects of Cur (100 mg · kg-1 · d-1ig) × 5 d alone and in combination with Gli (1 mg · kg-1 · d-1ig) Effects of Fasting Blood Glucose, Plasma Insulin, Insulin Sensitivity Index, Hepatic Glycogen, Liver Toxicity Index and CYP3A Activity in Type 2 Diabetic Rats. Results: Cur could further enhance the hypoglycemic effect of Gli. After treatment with Cur, the fasting blood glucose (22% -69%) and the insulin and glycogen level (49%, 19% -126%, 81% ). Cur can significantly reduce liver injury index, has a protective effect on the liver. Cur alone or in combination with Gli significantly inhibited CYP3A activity. CONCLUSION: Cur can enhance the hypoglycemic effect of Gli, which may be related to its inhibition of the activity of Gli metabolizing enzyme-CYP3A and the delay of Gli metabolism, resulting in its hypoglycemic effect.