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以动情前期大鼠垂体对ＧｎＲＨ的激发效应为模型，观察不同构型的甾体避孕药－抗孕５３和１８甲基炔诺酮（ＬＮＧ）对大鼠垂体功能的反应。电刺激视前区（ＰＯＡ）显示抗孕－５３组ＬＨ峰高于对照组（Ｐ＜０．０５），而ＬＮＧ组则显著低于对照组（Ｐ＜０．０１），表明抗孕－５３的增强效应和ＬＮＧ的抑制效应的关键部位在垂体前叶。若对经避孕药处理后的动情前期大鼠作静脉注射蛋白抑制剂放线菌酮（Ｃｙｃｌｏｈｅｘｉｍｉｄｅ）１００μｇ／１００ｇｂ．ｗ．，则抗孕－５３＋Ｃｙｃｌｏｈｅｘｉｍｉｄｅ组或Ｃｙｃｌｏｈｅｘｉｍｉｄｅ组均低于对照组（Ｐ＜０．０１），但抗孕－５３＋Ｃｙｃｌｏｘｉｍｉｄｅ组仍高于Ｃｙｃｌｏｈｅｘｉｍｉｄｅ组（Ｐ＜０．０５）。Ｃｙｃｌｏｈｅｘｉｍｉｄｅ组和ＬＮＧ＋Ｃｙｃｌｏｈｅｘｉｍｉｄｅ组相比前者比后者稍有下降，但无显著性差异。若对ＬＮＧ处理后的大鼠静脉注射纳洛酮（０．２５ｍｇ／１００ｇｂ．Ｗ）结果显示纳洛酮可解除ＬＮＧ的抑制效应。以上结果表明在一定剂量下抗孕－５３引起的增强垂体激发效应，可能不完全是增加ＧｎＲＨ分泌量，而主要是通过加强蛋白的合成来完成。ＬＮＧ的抑制垂体激发反应不是抑制ＧｎＲＨ的分泌而是通过垂体与阿片肽?
The anterior pituitary gland stimulation of GnRH was used as a model to observe the response of different steroid contraceptives - anti-pregnant 53 and 18 norethisterone (LNG) to pituitary function in rats. The electrical stimulation of the preoptic area (POA) showed that the LH peak in the anti-pregnant -53 group was significantly higher than that in the control group (P <0.05), while that in the LNG group was significantly lower than that in the control group (P <0.01) The enhancement effect and LNG inhibitory effect of the key site in the anterior pituitary. If the contraceptives after treatment of pre-estrous rats for intravenous protein inhibitor cycloheximide (Cycloheximide) 100μg / 100gb. w. (P <0.01), however, the levels of IL-53 + Cycloheximide group and Cycloheximide group were still lower than those of Cycloheximide group (P <0.05). Cycloheximide group and LNG + Cycloheximide group compared with the former slightly decreased, but no significant difference. The intravenous injection of naloxone (0.25 mg / 100 gb.W) to LNG-treated rats showed that naloxone could release the inhibitory effect of LNG. The above results show that anti-pregnancy -53-induced enhancement of pituitary stimulation at a certain dose may not completely increase GnRH secretion, but mainly by enhancing protein synthesis. Inhibition of LNG pituitary stimulation reaction is not to inhibit the secretion of GnRH but through the pituitary and opioid peptide?