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目的 探讨一氧化氮 (Nitricoxide,NO)在全胃肠外营养 (TotalParenteralNutrition ,TPN)导致正常肝脏及肝硬化大鼠肝损害中的影响。方法 30只正常Wistar大鼠和 30只肝硬化Wistar大鼠各自分别随机分为 5组 :A组 ,自由进食和水 ;B组 ,TPN ;C组 ,TPN +精氨酸 ;D组TPN +NG 硝基 L 精氨酸甲酯 (NG nitrio L argininemethylester,L NAME) ;E组 ,TPN +精氨酸 +L NAME。实验 7d后测肝功能、肝脂含量、肝脏NO含量、肝脏一氧化氮合酶 (NOsynthase ,NOS)活性和肝脏组织学检查。结果肝硬化大鼠肝脂和转氨酶B组比A组高 (P <0 0 5 ) ,C组比B组低 (P <0 0 5 ) ,D组比B组高 (P <0 0 5 ) ,D、E组较C组高 (P <0 0 5 ) ,E组较D组低 (P <0 0 5 )。正常大鼠各组间肝功能差异无显著意义 (P >0 0 5 ) ,正常组和肝硬化组的肝脂TG ,A组分别为 (6 9± 0 8) μmol/g、(9 1± 0 9) μmol/g ,D组为(4 9 7± 6 2 ) μmol/g、(34 2± 2 8) μmol/g ,C组为 (18 2± 4 4) μmol/g、(16 7± 2 7) μmol/g ,CHO变化与TG相一致 ,且与NO含量呈负相关。结论 NO能减轻TPN引起的肝损害。
Objective To investigate the effect of nitric oxide (NO) on liver damage in rats with normal liver and liver cirrhosis caused by total parenteral nutrition (TPN). Methods Thirty normal Wistar rats and thirty cirrhotic Wistar rats were randomly divided into five groups: group A, fed freely and water; group B, TPN; group C, TPN + arginine; group D TPN + NG NG nitrio L arginine methylester (L NAME); group E, TPN + arginine + L NAME. Liver function, hepatic lipid content, liver NO content, liver NO synthase (NOS) activity and liver histology were measured after 7 days. Results The levels of hepatic lipids and aminotransferases in cirrhotic rats were significantly higher than those in group A (P <0.05), those in group C were significantly lower than those in group B (P <0.05), those in group D were significantly higher than those in group B (P <0.05) (P <0.05) in group D and group E, and lower in group E than in group D (P <0.05). There was no significant difference in the liver function among the normal rats (P> 0.05). The hepatic TG and A in the normal group and the cirrhosis group were (69 ± 0 8) μmol / g, (91 ± (9 2 ± 2) μmol / g, (34 2 ± 2 8) μmol / g in group D, (18 2 ± 4 4) μmol / g in group C and ± 2 7) μmol / g. The change of CHO was consistent with TG and negatively correlated with NO content. Conclusion NO can reduce the liver damage caused by TPN.