以苍耳柄锈菌三裂叶豚草专化型Puccinia xanthii f.sp.ambrosiae-trifidae为研究试材,比较研究了其冬孢子DNA提取的9种方法,其中CTAB-钢珠法、改良的微型电钻法以及EZ-Kit改良法获得的基因组DNA经检测质量较好。在此基础上,利用ITS-PCR和ISSR引物UBC#835将待用DNA进行PCR扩增检测。结果表明,上述3种方法提取的DNA适合于ISSR反应。研究结果为专性寄生锈菌分子遗传变异的研究提供了保障。 Puccinia xanthii f.sp.ambrosiae-trifidae was used as experimental material, and 9 methods of DNA extraction from teliospore were studied. Among them, CTAB-ball method, modified miniature Genomic DNA obtained by the electric drill method and the EZ-Kit modification method is of good quality. On this basis, ITS-PCR and ISSR primer UBC # 835 were used for PCR amplification of DNA to be detected. The results showed that DNA extracted by the above three methods was suitable for ISSR reaction. The results provide a guarantee for the research on molecular genetic variation of obligate parasitic rust.