论文部分内容阅读
目的：为了研究聚乳酸和无活性脱钙骨基质载体对ｒｈＢＭＰ－２异位诱导的骨形成。方法：将重组人骨形态发生蛋白－２（ｒｈＢＭＰ－２）与无活性脱钙骨基质（ＤＢＭ）和聚乳酸（ＰＬＡ）复合，植入小鼠股部外侧肌肉陷窝比较ＰＬＡ和ＤＢＭ载体对ｒｈＢＭＰ－２诱导骨形成的影响。通过组织学检查、碱性磷酸酶（ＡＬＰ）和钙含量测定以及骨钙素（ＢＧＰ）放疫测定，进行对比观察。结果：术后２１天，实验组均可见新生软骨细胞和软骨岛，ｒｈＢＭＰ－２／ＰＬＡ和ＤＢＭ／ｒｈＢＭＰ－２组还可见部分骨组织形成，见骨小梁、板层结构和红骨髓，ｒｈＢＭＰ－２／ＰＬＡ组ＰＬＡ部分吸收，ＤＢＭ／ｒｈＢＭＰ－２组ＤＢＭ无吸收迹象。各实验组与对照组对比，ＡＬＰ、ＢＧＰ水平和Ｃａ量均有显著性差异（Ｐ＜０．０５），ｒｈＢＭＰ－２／ＰＬＡ与ＤＢＭ／ｒｈＢＭＰ－２组在ＡＬＰ和ＢＧＰ水平上相比无显著差异（Ｐ＞０．０５），但前组比后组的Ｃａ量为高（Ｐ＜０．０５），这二组与单独植入ｒｈＢＭＰ－２组相比，定量检测指标均有显著性差异（Ｐ＜０．０５）。结论：（１）ＰＬＡ和ＤＢＭ均为ＢＭＰ的良好载体；（２）ｒｈＢＭＰ－２／ＰＬＡ是一种新的有价值的骨修复材料
OBJECTIVE: To investigate the ectopic-induced bone formation of rhBMP-2 by polylactic acid and inactive decalcified bone matrix carriers. Methods: Recombinant human bone morphogenetic protein-2 (rhBMP-2) was mixed with inactive DBM and PLA and implanted into the lateral muscle fossa of mice. -2 induced bone formation. The histological examination, determination of alkaline phosphatase (ALP) and calcium content, and osteocalcin (BGP) were carried out to compare the results. RESULTS: On day 21 postoperatively, both newborn chondrocytes and cartilaginous island were observed in the experimental group. Partial bone formation was also observed in the rhBMP-2 / PLA and DBM / rhBMP-2 groups, with trabecular bone, lamellar structure and red bone marrow, rhBMP -2 / PLA PLA part of the absorption, DBM / rhBMP-2 DBM no signs of absorption. The levels of ALP, BGP and Ca in experimental group and control group were significantly different (P <0.05), but there was no significant difference in ALP and BGP between rhBMP-2 / PLA and DBM / rhBMP-2 (P> 0.05), but the Ca content of the former group was higher than that of the latter group (P <0.05). Compared with rhBMP-2 alone group, the quantitative detection indexes of these two groups were significantly different (P <0.05). Conclusions: (1) Both PLA and DBM are good carriers for BMP. (2) rhBMP-2 / PLA is a new valuable bone repair material