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目的:探讨积雪甙治疗伤口愈合的影响。方法:采用MTT法测量积雪甙对角质形成细胞和血管内皮细胞株增殖作用的影响,采用RT-PCR方法测量积雪甙对血管内皮细胞分泌KGF- 2mRNA量的影响。结果:积雪甙在浓度为1.5μg/ml左右时对角质形成细胞和血管内皮细胞促增殖作用最大,在此浓度下血管内皮细胞KGF-2mRNA表达量最大。结论:积雪甙在浓度为1.5μg/ml对伤口愈合促进作用最大。
Objective: To investigate the effect of asiaticoside on wound healing. Methods: The effect of asiaticoside on the proliferation of keratinocytes and vascular endothelial cell lines was measured by MTT method. The effect of asiaticoside on the secretion of KGF-2 mRNA by endothelial cells was measured by RT-PCR. Results: Asiaticoside had the strongest effect on the proliferation of keratinocytes and vascular endothelial cells at the concentration of 1.5μg / ml, and the highest expression of KGF-2mRNA was found in this concentration. CONCLUSION: Asiaticoside has the greatest effect on wound healing at a concentration of 1.5μg / ml.