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利用正交试验进行了丁香的幼胚离体培养和增殖培养两方面的研究,对基本培养基、糖浓度、谷氨酰胺浓度、BA浓度、NAA浓度都进行了仔细的筛选。结果表明:幼胚培养的最适胚龄为50天;幼胚培养所需要的最适培养基为附加BA(0.1mg稬-1)、NAA(0.001mg稬-1),糖50(g稬-1),Gln(400mg稬-1)的Monnier培养基,幼胚萌发率可达91.7%;Gln的加入对幼胚培养有显著影响。此外,丁香实生苗最适的增值培养基为MS+BA(2mg稬-1)、NAA(0.01mg稬-1)、IBA(0.5mg稬-1)、Gln(100mg稬-1),此时繁殖系数大于3.6,可以满足生产需要。图1表7参8。
The orthogonal test was used to study the in vitro and in vitro culture of clove embryos. The basic medium, sugar concentration, glutamine concentration, BA concentration and NAA concentration were carefully screened. The results showed that the optimum embryo age for immature embryo culture was 50 days. The optimum medium for immature embryo culture was BA (0.1 mg 稬 -1), NAA (0.001 mg 稬 -1) and sugar 50 -1), Gln (400mg 稬 -1) in Monnier medium, the embryo germination rate was 91.7%; Gln addition had a significant effect on immature embryo culture. In addition, the optimum medium for growth of clove seedlings was MS + BA (2mg 稬 -1), NAA (0.01mg 稬 -1), IBA (0.5mg 稬 -1) and Gln (100mg 稬 -1) Breeding coefficient greater than 3.6, to meet production needs. Figure 1 Table 7 参 8.