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用微注射法将不同浓度的细胞分裂素(激动素,KT)引入开花后30天的大豆种子胚与种皮间的质外体空间,同时给叶片饲喂~(14)CO_2,以种皮和子叶中放射强度占总放射强度的百分比来进行比较分析KT对同化物卸出的影响,结果表明;10~(-4)、10~(-5)mol/L的KT促进种皮中同化物的卸出和向子叶中的分配。同时测定了种皮和胚中的酸性转化酶和ATP酶的活性,KT使种皮中酸性转化酶活性增加,而ATP酶活性降低,由此促进种皮同化物的卸出;胚中的ATP酶活性增加,提供更多的能量,有利于对糖的主动吸收。分析了胚中的糖和蛋白质的转化及蛋白酶的活性,看出KT对胚代谢产生了影响,但是情况较为复杂,尚未明确其内在联系。
Different concentrations of cytokinin (KT) were introduced into the apoplast space of soybean seed germ and seed coat at 30 days after flowering by microinjection method, while the leaves were fed with ~ (14) CO_2, And the percentage of radiation intensity in the cotyledons to the total radiation intensity were compared to analyze the effect of KT on the unloading of the assimilates. The results showed that 10 ~ (-4) and 10 ~ (-5) mol / L KT promoted the assimilation Discharge of material and distribution to cotyledons. At the same time, the activities of acid invertase and ATPase in seed coat and embryo were determined. KT increased the activity of acid invertase in the seed coat and decreased the activity of ATPase, thereby promoting the unloading of seed coat assimilate. The ATP Enzyme activity increases, providing more energy, is conducive to the active absorption of sugar. The transformation of carbohydrate and protein in embryos and the activity of protease were analyzed. It was found that KT had an impact on embryo metabolism, but the situation is more complicated, and its intrinsic relationship has not yet been clarified.