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目的：了解2012-2013年黄山地区流行的肠道病毒71型（EV71）基因型情况。方法：利用RT-nPCR检测两年43份临床诊断为手足口病患儿的咽拭子标本。结果：43份标本中检测到13份阳性EV71 RNA，检出率为30.23%；经过测序证实13份标本均为EV71 C4型。结论：黄山地区2012-2013年手足口病主要由C4型 EV71病毒引起且存在很高的同源性。“,”[Abstact] Objective In order to investigate the EV71 genotypes in Huangshan area. Methods EV71 RNA was detected in 43 HFMD samples from the hospitals by reverse translation polymerase chain reaction(RT- nPCR). Result There are 13 positive products, positive rate is 30.23%;After the 13 positive products were sequenced, the results show them al C4 genotype EV71. Conclusion It seems that the HFMD children are mainly infected by the C4 genotype EV71 in this area and the current C genotype EV71 did not varied significantly in the city.