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目的研究成釉细胞瘤(ameloblastoma,AB)中 hTERT 启动子区的 DNA 甲基化,并探讨其生物学意义。方法选取新鲜标本 AB 12例,同时用11例正常黏膜做对照观察,用甲基化特异PCR 检测上述组织中人类端粒酶反转录酶(human telomerase reverse transcripase,hTERT)启动子区的DNA 甲基化。结果 AB、正常黏膜中 hTERT 启动子区的 DNA 非甲基化阳性分别为4例(4/12)和6例(6/11)。AB、正常黏膜中 hTERT 启动子区的 DNA 甲基化阳性分别为11例(11/12)和3例(3/11),其中4例 AB 和1例正常黏膜同时表现 hTERT 启动子区 DNA 的甲基化和非甲基化。结论AB 的 hTERT 启动子区的 DNA 甲基化比正常黏膜常见且有意义;hTERT 启动子的甲基化可能对hTERT 基因起调节作用。
Objective To study the DNA methylation of hTERT promoter in ameloblastoma (AB) and to explore its biological significance. Methods Fresh samples were collected from 12 patients with AB, and 11 normal mucosa were also used as controls to detect DNA methylation in human telomerase reverse transcriptase (hTERT) promoter region by methylation-specific PCR Based on. Results There were 4 cases (4/12) and 6 cases (6/11) of non-methylation of hTERT promoter in normal mucosa. AB, the methylation of hTERT promoter region in normal mucosa were 11 (11/12) and 3 (3/11), respectively, in which 4 cases of AB and 1 case of normal mucosa simultaneously expressed the DNA of hTERT promoter Methylation and unmethylation. Conclusion DNA methylation of hTERT promoter in AB is more common and more significant than normal mucosa. Methylation of hTERT promoter may play a regulatory role in hTERT gene.