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An analytical method for quantitative estimation of the cytotoxicity of dental alloys is introduced. After the TC50 values of 9 metal cations of various dental casting alloys were established using the MTT assay and the concentrations of the corosively dissolved metal cations in the alloy extracts were measured by means of an ICP method. The cytotoxicity of dental alloys is induced as a function of the TC50 values and concentrations. The validity of the method is testified, by comparing the effective cytotoxicity of 19 dental alloys with those obtained directly by using MTT assay.
An analytical method for quantitative estimation of the cytotoxicity of dental alloys is introduced. After the TC50 values of 9 metal cations of various dental casting alloys were established using the MTT assay and the concentrations of the corosively dissolved metal cations in the alloy extracts were measured by means of an ICP method. The cytotoxicity of dental alloys is induced as a function of the TC50 values and concentrations. The validity of the method is testified, by comparing the effective cytotoxicity of 19 dental alloys with those obtained directly by using MTT assay.