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目的探讨δ-氨基乙酰丙酸脱水酶(δ-aminolevulinic acid dehydratase,ALAD)基因多态性对职业铅暴露工人血铅和血红蛋白水平的影响。方法选择武汉市某铅蓄电池厂187名职业铅暴露工人,石墨炉原子吸收光谱法测定血铅水平,氰化高铁法检测血红蛋白含量,聚合酶链反应-限制性片段长度多态性方法检测ALAD基因亚型,限制性内切酶为MspⅠ。结果 187名职业铅暴露工人中,ALAD11纯合子基因型175人,占93.58%,ALAD12杂合子基因型12人,占6.42%,未检测到ALAD22纯合子基因型;ALAD12基因型铅暴露工人血铅浓度高于ALD11基因型的工人,但差异无统计学意义(t=-0.47,P=0.640);ALAD11基因型工人的血红蛋白浓度高于ALAD12基因型工人,差异有统计学意义(t=3.31,P=0.004)。结论 ALAD12基因型工人较ALAD11基因型对铅的血液毒性更易感。
Objective To investigate the effect of δ-aminolevulinic acid dehydratase (ALAD) gene polymorphism on blood lead and hemoglobin levels in workers exposed to lead. Methods A total of 187 lead exposure workers in a lead-acid battery factory in Wuhan were determined. Blood lead levels were determined by graphite furnace atomic absorption spectrometry. The hemoglobin content was detected by cyanidation and the ALAD gene was detected by polymerase chain reaction-restriction fragment length polymorphism Subtype, restriction enzyme MspI. Results Among 187 lead exposure workers, 175 were ALAD11 homozygous (93.58%), 12 were ALAD12 heterozygous (6.42%), ALAD22 homozygous genotype was not detected, and ALAD12 genotype lead exposure (T = -0.47, P = 0.640). The hemoglobin concentration of ALAD11 genotype workers was higher than that of ALAD12 genotype workers (t = 3.31, P = 0.004). Conclusion ALAD12 genotype workers are more susceptible to lead toxicity than ALAD11 genotypes.