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目的:观察β-七叶皂苷(β-aescin)对大鼠脊髓损伤后继发性损害的保护作用。方法:本实验采用NYUⅡ型脊髓撞击伤仪制成中度脊髓损伤模型。术后30 min,所有大鼠分别经腹腔给予β-七叶皂苷(1.0 mg/kg)或盐水治疗,3次/d,连给3 d。术后,于各时间点采用BBB评分和足迹实验对大鼠双后肢运动功能进行评估;术后24 h,对损伤部位脊髓组织丙二醛(MDA)含量及髓过氧化物酶(MPO)活性进行检测;术后14 d行免疫组织化学检查,利用胶质原纤维酸性蛋白(GFAP)观察损伤星形胶质瘢痕及其包围的坏死空洞,利用抗CD68/ED1观察活性小胶质细胞和巨噬细胞。结果:β-七叶皂苷组运动功能明显改善(P<0.05);伤后24 h,β-七叶皂苷组大鼠脊髓组织中MDA水平降低67%(P<0.01),MPO活性降低50%(P<0.01);伤后14 d,β-七叶皂苷组空洞面积减小29.3%(P<0.01),ED1染色阳性面积减少32.3%(P<0.01)。结论:β-七叶皂苷治疗可以减轻脊髓损伤后自由基的氧化损伤作用,减小空洞面积,减少炎症细胞的活化和浸润,促进脊髓损伤后的修复。
Objective: To observe the protective effect of β-aescin on secondary damage after spinal cord injury in rats. Methods: In this study, a moderate spinal cord injury model was made by NYU type Ⅱ spinal cord injury device. At 30 min after operation, all rats were treated with β-aescinate (1.0 mg / kg) or saline intraperitoneally, 3 times / d for 3 days. At the same time, BBB score and footprinting were used to evaluate the motor function of both hindlimbs at each time point. The content of malondialdehyde (MDA) and the activity of myeloperoxidase (MPO) The immunohistochemistry was performed 14 days after operation. GFAP was used to observe the damage of astroglial scar and its surrounding necrotic cavity. Anti-CD68 / ED1 was used to observe the activity of microglial cells and macrophages Phagocytic cells. RESULTS: The motor function of β-aescinate group was significantly improved (P <0.05). At 24 h after injury, the level of MDA in spinal cortex decreased by 67% (P <0.01) and the activity of MPO decreased by 50% (P <0.01). On the 14th day after injury, the area of β-aescin group decreased by 29.3% (P <0.01), and the positive area of ED1 staining decreased by 32.3% (P <0.01). CONCLUSION: The β-aesculin treatment can reduce the oxidative damage of free radicals after spinal cord injury, reduce the void area, reduce the activation and infiltration of inflammatory cells and promote the repair after spinal cord injury.