论文部分内容阅读
用异硫氰酸荧光素(FITC)标记EB病毒(EBV)感染体外培养的人胚鼻咽上皮和鼻咽癌细胞株(CNE-1和CNE-2),对照为EBV受体阳性的Raji和Ramos细胞。在荧光显微镜下发现FITC-EBV 可与Raji和Ramos细胞结合,如事先经未标记的EBV处理则与 FITC-EBV 结合的阳性细胞数明显下降。贴壁生长的鼻咽上皮细胞不能与FITC-EBV 结合,而刮落的鼻咽上皮细胞则能与FITC-EBV 结合,结果提示鼻咽上皮细胞质膜微细损伤可能成为EBV 直接进入细胞的门户,受损的鼻咽癌细胞更易为EBV 侵入。
The human embryonic nasopharyngeal epithelial and nasopharyngeal carcinoma cell lines (CNE-1 and CNE-2) cultured in vitro were infected with fluorescein isothiocyanate (FITC) labeled Epstein-Barr virus (EBV), and the control was EBV receptor positive Raji and Ramos cells. FITC-EBV was found to bind to Raji and Ramos cells under a fluorescence microscope. The number of positive cells bound to FITC-EBV was significantly reduced as previously untreated EBV. Adherent nasopharyngeal epithelial cells can not bind to FITC-EBV, while scraped nasopharyngeal epithelial cells can bind to FITC-EBV. The results suggest that the plasma membrane of nasopharyngeal epithelial cells may become the gateway to EBV directly into cells Damaged nasopharyngeal carcinoma cells more easily EBV invasion.