论文部分内容阅读
选用成年、健康杂种犬15只,术前经超声多普勒测量门静脉血流量,个体间采用自身对照。经开腹术穿刺门静脉测压,分次注入白芨粉混悬液,使门脉压升高至3.43 kPa(35 cmH_2O)以上,术后10天、20天,60天重复注射白芨液,术后100天犬门脉血流动力学出现如下变化:门脉压力由术前1.59±0.26 kPa增至3.09±0.49 kPa(31.52±4.97 cmH_2O)。胃镜及病理切片证实食管下段粘膜静脉曲张形成,汇管区出现纤维化。门静脉血流量由术前449.41±84.12 ml/min增至565.72±105.93 ml/min。白芨粉无任何毒性,注入血管可产生确切的人工血栓。制备犬门脉高压症模型简单,存活率高,周期短,可望用于基础及临床研究。
Fifteen adult and healthy mongrel dogs were selected, and portal vein blood flow was measured by Doppler before surgery. Self-control was used among individuals. The portal vein pressure was measured by laparotomy. The portal vein pressure was increased to 3.43 kPa (35 cm H 2 O) or more after the portal vein pressure was divided into several sub-groups. Bletilla striata was injected 10 days, 20 days and 60 days after operation. The changes of portal hemodynamics in 100 days were as follows: portal pressure increased from 1.59 ± 0.26 kPa to 3.09 ± 0.49 kPa (31.52 ± 4.97 cmH 2 O) before surgery. Gastroscopy and pathology confirmed lower esophageal mucosal varices, portal area fibrosis. Portal vein blood flow increased from 449.41 ± 84.12 ml / min preoperatively to 565.72 ± 105.93 ml / min. Bletilla striata without any toxicity, into the blood vessels can produce the exact artificial thrombosis. Preparation of canine portal hypertension model is simple, high survival rate, short cycle, expected to be used for basic and clinical studies.