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目的采用多普勒彩超辐照不同孕期大鼠,观察超声对胎鼠脑组织超微结构的影响。方法采用多普勒彩超照射不同孕期大鼠(孕12d、15d、18d)30min,仪器采用PREIRUS型彩色电脑声像仪,照射条件:EUP-LTM4探头,二维频率5.0 MHz,组织热指数(Tis)=0.4,机械指数(Micd)=1.2。照射后24h取胚胎脑组织标本,利用透射电镜(TEM)观察细胞器超微结构变化。结果经过多普勒彩超辐照30min后,孕期12d胎鼠神经元细胞器超微结构改变不明显,偶见线粒体嵴溶解,内质网未见明显扩张,细胞核形状较规则,胞质中有较小的空泡出现;孕期15d胎鼠神经元细胞器超微结构改变明显,细胞器损伤程度重于12d胎鼠;孕期18d胎鼠可见明显超微结构改变,内质网明显扩张,细胞核形态严重不规则,线粒体嵴溶解更明显,核周间隙扩张,胞质中出现较大空泡。结论多普勒彩超辐照对不同孕期胎鼠脑组织中细胞器超微结构的影响不同,胎鼠孕期越早,相同的辐照对其影响越小。
Objective To observe the effect of ultrasound on the ultrastructure of fetal rat brain tissue by using Doppler ultrasound to illuminate different pregnant rats. Methods Doppler ultrasound was used to irradiate different pregnant rats (pregnant 12d, 15d, 18d) for 30min. The instruments were equipped with PREIRUS color computer imager. The irradiation conditions were EUP-LTM4 probe, two dimensional frequency 5.0 MHz, Tissue thermal index ) = 0.4, Mechanical Index (Micd) = 1.2. Embryonic brain tissue samples were taken 24 hours after irradiation, and ultrastructure changes of organelles were observed by transmission electron microscopy (TEM). Results After Doppler ultrasound irradiation for 30 min, the changes of ultrastructure of organelles in fetal rat neurons were not obvious after 12 d of gestation. Occasionally mitochondrial cristae were dissolved and there was no obvious expansion in endoplasmic reticulum. The shape of nucleus was regular and the cytoplasm was smaller The vacuoles of embryonic rats appeared obviously; the ultrastructure of organelles of fetal rat neurons changed obviously during pregnancy, and the degree of organelle injury was heavier than that of 12 d embryos. The fetal ultrastructural changes were observed on 18 d of pregnancy, the endoplasmic reticulum was obviously expanded and the morphology of nucleus was serious and irregular, Mitochondrial cristae dissolved more obvious perinuclear space expansion, the cytoplasm larger vacuoles. Conclusions Doppler ultrasound has different effects on the ultrastructure of organelles in different stages of fetal rat embryos. The earlier the embryo is during pregnancy, the smaller the effect of the same irradiation is.