A non-damage method for measuring local tissue blood perfusion rate by surface heat disturbance and its two particular embodimellts for realizing this measurement are presented in this paper. In derivation of the mathematic model, the Pennes equation is used, and two parameters-the arterial blood temperature Ta and the metabolic heat generation rate qm which are very difficult to be known their values have been eliminated. The effectiveness of the measurement method has been demonstrated by means of the quantitative measurement of tissue in vitro, the comparative measurement of animal under artificial perfusion and the dependence measurement in humam body. A non-damage method for measuring local tissue blood perfusion rate by surface heat disturbance and its two particular embodimellts for realizing this measurement are presented in this paper. In derivation of the mathematic model, the Pennes equation is used, and two parameters-the arterial blood temperature Ta and the metabolic heat generation rate qm which are very difficult to be known their values ​​have been eliminated. The effectiveness of the measurement method has been demonstrated by means of the quantitative measurement of tissue in vitro, the comparative measurement of animal under artificial perfusion and the dependence measurement in humam body.