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用双苯并咪唑(Hoechst33258)染色法分别对长根小奥德蘑Oudemansiella radicata双孢菌株和四孢菌株的菌丝、子实体、担孢子进行染色观察,结果表明:双孢长根小奥德蘑菌丝细胞多为单核,无锁状联合;原担子中单核进行一次有丝分裂形成两个横向或纵向排列的子核,这2个子核分别进入2个担孢子中,留下无核的空担子;成熟担孢子具有一个核。四孢长根小奥德蘑菌丝细胞大多数为双核,具有锁状联合;进入原担子中的两个单倍性细胞核先发生核配,形成一个二倍性的核,再经过减数分裂形成四个染色体减半的单倍性子核,4个子核分别进入4个担孢子中,留下无核的空担子;成熟担孢子也具有一个核。对长根小奥德蘑双孢子实体形成的机理进行了推测。
The mycelia, fruiting bodies and basidiospores of Oudemansiella radicata and Corynebacterium sp. Were stained with bisbenzimidazole (Hoechst 33258) staining, respectively. The results showed that: Mushroom cells mostly mononuclear, no lock-like combination; the original burden of a single nuclear mitosis to form two transverse or longitudinal arrangement of sub-nuclei, the two sub-nucleus into two basidiospores, leaving a nuclear-free Empty burden; mature basidiospore with a core. Most of the tetradosporidium root cells are dual-nuclei, with a lock-like combination; into the original burden of the two haploidentical nuclei first nuclear distribution, the formation of a diploid nucleus, and then after meiosis Four haploid nuclei formed in half of the chromosomes were formed, and four sub-nuclei entered into four basidiospores, respectively, leaving a nuclear-free empty burden. The mature basidiospores also had a nuclear. The mechanism of the formation of Entomoides arundinacea was inferred.